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zurich viral core v923 dj aav hsyn1 sio eopn3 mscarlet wpre aav1 addgene 125713 aav1 experimental models  (Addgene inc)


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    Addgene inc zurich viral core v923 dj aav hsyn1 sio eopn3 mscarlet wpre aav1 addgene 125713 aav1 experimental models
    Zurich Viral Core V923 Dj Aav Hsyn1 Sio Eopn3 Mscarlet Wpre Aav1 Addgene 125713 Aav1 Experimental Models, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+dj+-hsyn-egfp/pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE+(Plasmid+%23125713)/pm41763211-230-95-101
    Average 93 stars, based on 24 article reviews
    zurich viral core v923 dj aav hsyn1 sio eopn3 mscarlet wpre aav1 addgene 125713 aav1 experimental models - by Bioz Stars, 2026-10
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    Related Articles

    Bioprocessing:

    Article Title: Anxa1+ dopamine neuron vulnerability defines prodromal Parkinson’s disease bradykinesia and procedural motor learning impairment
    Article Snippet: For optogenetic experiments, 300 nL of AAV8-nEF-Coff/Fon-ChRmine-oScarlet (Cat# 137160, Addgene) was bilaterally injected into Anxa1-flp mice, while 300 nL of AAV8-nEF- Con/Foff 2.0-ChRmine-oScarlet (Cat# 137161, Addgene) was injected into DAT-Cre mice at the same coordinates. .. For FP recordings, unilateral injections of 300 nL or 500 nL AAV-DJ/2-hSyn1-chl-dFRT-jCaMP8m(rev)-dFRT-WPRE-bGHp (v740-DJ/22887, VVF Zurich) were administered to Anxa1-flp mice, while 300 nL of pGP-AAV9-CAG-FLEX-jGCaMP8m-WPRE (Cat# 162381, Addgene) was used in DAT-Cre mice. .. DA release recordings were conducted by injecting 500 nL of AAV5-CAG-dLight1.3b (Cat# 125560, Addgene) unilaterally into C57BL6 mice.

    Article Title: TTP protects against acute liver failure by regulating CCL2 and CCL5 through m 6 A RNA methylation
    Article Snippet: .. Briefly, HEK293T cells (ATCC) were transfected with AAV-DJ (Addgene, catalog 130878), px552 (Addgene, catalog 60958), and AAV-helper (Addgene, catalog 81070) plasmids in 150 mm dishes at 80% confluence. ..

    Article Title: MCM7 promotes liver fibrosis by transcriptionally regulating IL11 via the SHCBP1-RACGAP1-STAT3 axis
    Article Snippet: .. HEK293T cells were transfected with the AAV-shCtrl, AAV-DJ (Addgene; Cat No.130878), and AAV-helper (Addgene; Cat No.81070) plasmids. ..

    Article Title: KHSRP ameliorates acute liver failure by regulating pre-mRNA splicing through its interaction with SF3B1
    Article Snippet: .. HEK293T cells were transfected with PX552, AAV-DJ (Addgene; catalog no. 130878), and AAV-helper (Addgene; catalog no. 81070) plasmids. ..

    Transfection:

    Article Title: TTP protects against acute liver failure by regulating CCL2 and CCL5 through m 6 A RNA methylation
    Article Snippet: .. Briefly, HEK293T cells (ATCC) were transfected with AAV-DJ (Addgene, catalog 130878), px552 (Addgene, catalog 60958), and AAV-helper (Addgene, catalog 81070) plasmids in 150 mm dishes at 80% confluence. ..

    Article Title: MCM7 promotes liver fibrosis by transcriptionally regulating IL11 via the SHCBP1-RACGAP1-STAT3 axis
    Article Snippet: .. HEK293T cells were transfected with the AAV-shCtrl, AAV-DJ (Addgene; Cat No.130878), and AAV-helper (Addgene; Cat No.81070) plasmids. ..

    Article Title: KHSRP ameliorates acute liver failure by regulating pre-mRNA splicing through its interaction with SF3B1
    Article Snippet: .. HEK293T cells were transfected with PX552, AAV-DJ (Addgene; catalog no. 130878), and AAV-helper (Addgene; catalog no. 81070) plasmids. ..



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    A) Representative Western blot gels of the WT cerebral cortical neuron infected <t>with</t> <t>either</t> <t>AAV-DJ-GFP</t> or -P301S virus, as indicated. Molecular weights are indicated in kDa. B) Representative Western blot gels of the cerebral cortical neuron from WT and P301S embryos, as indicated. Molecular weights are indicated in kDa.
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    A) Representative Western blot gels of the WT cerebral cortical neuron infected <t>with</t> <t>either</t> <t>AAV-DJ-GFP</t> or -P301S virus, as indicated. Molecular weights are indicated in kDa. B) Representative Western blot gels of the cerebral cortical neuron from WT and P301S embryos, as indicated. Molecular weights are indicated in kDa.
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    Image Search Results


    Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers against AAV-DJ capsid proteins and the expressed P2C5-Fc antibody.

    Journal: Frontiers in Immunology

    Article Title: Long-term protective potency of AAV vector-based SARS-CoV-2 prophylaxis in mice and non-human primates

    doi: 10.3389/fimmu.2026.1829230

    Figure Lengend Snippet: Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers against AAV-DJ capsid proteins and the expressed P2C5-Fc antibody.

    Article Snippet: The rAAV-P2C5-Fc vector construct was produced using a three-plasmid AAV-DJ packaging system (Cell Biolabs, USA) by transient transfection of HEK293 cells (obtained from the cell collection of the N.F.

    Techniques: Concentration Assay, Activity Assay, Immunopeptidomics

    Eight embryos were injected in utero at E15.5 with a pooled barcoded library of seven capsids (AAV9, AAV-DJ, AAV1, AAV6, CAP-B10, PHP.eB, X1.1) at equal concentrations and collected at E18.5. Data were analyzed using linear mixed effects models with tissue, capsid, and normalized reads as fixed effects, and biological replicates as a random effect, with AAV9 set as the reference capsid. A-B) Normalized DNA (A) and RNA (B) abundance of each capsid in cortex, caudal brain, eyes, liver, heart, and lung, relative to AAV9, quantified by NGS. Brain-biased capsids (PHP.eB, CAP-B10, X1.1; tropism established in adult mice) showed no statistically significant enrichment in CNS tissues or depletion in liver (all p > 0.05). C-D) Normalized DNA (C) and RNA (D) abundance in gonads (ovaries and testes; n = 2 female, n = 6 male).

    Journal: bioRxiv

    Article Title: Defining a Midgestational Window for In Utero Genome Editing of the Fetal Murine Cortex

    doi: 10.64898/2026.04.28.721509

    Figure Lengend Snippet: Eight embryos were injected in utero at E15.5 with a pooled barcoded library of seven capsids (AAV9, AAV-DJ, AAV1, AAV6, CAP-B10, PHP.eB, X1.1) at equal concentrations and collected at E18.5. Data were analyzed using linear mixed effects models with tissue, capsid, and normalized reads as fixed effects, and biological replicates as a random effect, with AAV9 set as the reference capsid. A-B) Normalized DNA (A) and RNA (B) abundance of each capsid in cortex, caudal brain, eyes, liver, heart, and lung, relative to AAV9, quantified by NGS. Brain-biased capsids (PHP.eB, CAP-B10, X1.1; tropism established in adult mice) showed no statistically significant enrichment in CNS tissues or depletion in liver (all p > 0.05). C-D) Normalized DNA (C) and RNA (D) abundance in gonads (ovaries and testes; n = 2 female, n = 6 male).

    Article Snippet: Self-complementary pAAVs were generated from pscAAV-CAG-GFP, a gift from Mark Kay (Addgene, 83279). pAAV2/1 (gift from James M. Wilson; Addgene plasmid #112862), pAAV2/2 (gift from Melina Fan; Addgene plasmid #104963), AAV3 rep-cap (Cell Biolabs, VPK-423), AAV4 rep-cap (Cell Biolabs, VPK-424), pAAV2/5 (gift from Melina Fan; Addgene plasmid #104964), AAV6 rep-cap (Cell Biolabs, VPK-426), pAAV2/7 (gift from James M. Wilson; Addgene plasmid #112863), pAAV2/8 (gift from James M. Wilson; Addgene plasmid #112864), pUCmini-iCAP-AAV-PHP.eB13 (Addgene #103005), pUCmini-iCAP-AAV.CAP-B10 (Addgene #175004), AAV-DJ rep-cap (Cell Biolabs, VPK-420-DJ), and pUCmini-iCAP-AAV9-X1.1 (Addgene #196836) were used for production of AAVs together with the adenoviral helper plasmid pHelper (Agilent Technologies, #240071).

    Techniques: Injection, In Utero

    A) Representative Western blot gels of the WT cerebral cortical neuron infected with either AAV-DJ-GFP or -P301S virus, as indicated. Molecular weights are indicated in kDa. B) Representative Western blot gels of the cerebral cortical neuron from WT and P301S embryos, as indicated. Molecular weights are indicated in kDa.

    Journal: bioRxiv

    Article Title: p75 neurotrophin receptor signaling through the RhoA/ROCK pathway contributes to Alzheimer’s Disease tauopathy

    doi: 10.64898/2026.02.22.707328

    Figure Lengend Snippet: A) Representative Western blot gels of the WT cerebral cortical neuron infected with either AAV-DJ-GFP or -P301S virus, as indicated. Molecular weights are indicated in kDa. B) Representative Western blot gels of the cerebral cortical neuron from WT and P301S embryos, as indicated. Molecular weights are indicated in kDa.

    Article Snippet: AAV-DJ-GFP and -P301S virus were purchased from Vigene Biosciences.

    Techniques: Western Blot, Infection, Virus